Andrea Messina (messina@science.unitn.it)
Pubblicazioni
Place/date of birth: Portoferraio (Livorno), Italy; 16 luglio 1980
Address: Via Gocciadoro, 152 Trento
Nationality: Italian
Actual Position: Post-doctoral fellowship in CIBIO (University of Trento)
Knowledge of foreign languages: Italian and English
Academic records:
September-December, 2010: Teaching Laboratory Class in Physiology, University of Trento (Supervisor: Dr Yuri Bozzi).
September-December, 2009: Teaching Laboratory Class in Developmental Biology, University of Trento (Supervisor: Dr Simona Casarosa).
May 04, 2009: Post-Doctoral Fellowship at the Centre for Integrative Biology (CIBIO), University of Trento (Supervisor: Dr Simona Casarosa).
February 13, 2009: Ph.D. fellowship at the University of Siena in Cellular and Molecular Physiology, Pharmacology and Toxicology (Supervisor: Dr PierGiovanni Gervasi). Title of the project: “Presence and regulation of cytochrome P450 isoforms and oxidative stress enzymes in swine heart and nasal mucosa and cloning of new CYPs in this animal model.”
June, 2006: Qualification to practice Biologist profession. University of Pisa.
May 05, 2005: Graduate in Biology (107/110). University of Pisa. (Supervisor: Prof. Irma Nardi and Dr Michela Ori). Title of the project: “Characterization of XPet-1 expression in development of serotoninergic neurons of Xenopus laevis embrios”.
Professional Experiences:
September-December, 2010: Teaching Laboratory Class in Physiology, University of Trento (Supervisor: Dr Yuri Bozzi).
April 09-20, 2010: Cold Spring Harbor Laboratory Course: “Cell and Devolpment of Xenopus”. Cold Spring Harbor Laboratory. New York (NY).
September-December, 2009: Teaching Laboratory Class in Developmental Biology, University of Trento (Supervisor: Dr Simona Casarosa).
May 04, 2009: Post-Doctoral Fellowship at the Centre for Integrative Biology (CIBIO), University of Trento (Supervisor: Dr Simona Casarosa).
December 06, 2007 – April 12, 2009: Agreement grant between Abiogen Pharma and Institute of Clinical Physiology of CNR (Pisa, Italy). Title of the project: “Metabolic characterization of new molecoles with pharmacological activity”. (Supervisor: Dr. PierGiovanni Gervasi. Department of Pharmacogenetic and drug metabolism).
Professional compentences:
Animal Models:
- Swine
- Xenopus laevis
- Mouse
- Rat
- Sheep
Molecular Biology competence:
- RNA and DNA manipulation (restriction enzymes, RNA extraction by tissue and cell colture, genomic extraction, plasmid propagation and extraction, molecular cloning)
- Qualitative and semi-quantitive RT-PCR
- Real-Time PCR
- GeneRacer PCR
- Escherichia coli manipulation and colture
- Generation of chimeric protein
- In vitro probe transcription
- In vitro mRNA transcription
- Antibodies production
- Heterologous expression of mammalian protein in Escherichia coli
- Western Blot
- Microarray
Molecular embriology compentence:
- mRNA and morpholino microinjection in Xenopus laevis embrios (Experiment of gain and loss of function)
- In situ hybridation in Xenopus laevis and mouse tissues (whole mount and on section)
- Immunohistochemestry in Xenopus laevis and mouse tissues (whole mount and on section)
- Gene reporter (β-galattosidase, alcalin fosfatase, peroxidase, Green Fluorescent Protein)
Experimental embriology competence:
- Anphibian embriology (staging, microdissection and microinjection).
- Skeleton and cartilage staining in Xenopus laevis and mouse
- Retrograde labeling of Mauthner neurons in Xenopus laevis embrios
Biochemestry competence:
- CYP450 assay
- DT-diaphorasi and Glutathione-S-Transferase activities
- Antioxidant enzymes specific activity (Catalase, Superoxide Dismutase, Glutathion Reductase, Glutathion Peroxidase)
- HPLC
Cellur Biology competence:
- ESCs culture
- ESCs treatment and neural differentiation
- Tumorigenic cell culture
Instrument:
- Fluorescence microscope, microinjector, microtome, vibratome, cryostat, centrifuge, ultracentrifuge, spectrophotometer, Fluorimeter, HPLC, chemical hood, biological hood
Informatics:
- Blast, Clustal, Strider algorithms
- Photoshop, PowerPoint, Exell
- Internet database analyses
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