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Laboratory of Medical Genetics
The Medical
Genetics Unit, located at 1st floor
3rd block of Policlinico S. Maria alle Scotte, viale Bracci 2,
Siena, has
approximately 530 sq. m. available, including laboratory spaces
(approximately 422 sq. m.)., two ambulatories for
genetic counselling (approximately 57 sq. m.) and two offices
(approximately 51sq. m.). The laboratory spaces include a total of
20 workstations and the offices include a total of 15 computer
stations. The Medical Genetics laboratory
is equipped with the major items required for molecular biology
activities. In particular there are: a nucleic acids extractor (Qiagen
EZ1 Advanced), three PCR machines (one Perkin Elmer GeneAmp PCR
System 2400, one Biometra T3 Thermocycler with 3 independent blocks
and one BIORAD MJ Mini), a real−time quantitative PCR machine
(Applied Biosystem ABIPrism 7000 Sequence Detection System), two
stations for DHPLC analysis (Transgenomics WAVE 1100 DNA fragment
Analysis System), two machines for automated fluorescent DNA
sequencing (Perkin Elmer ABI310 Genetic Analyzer), a machine for
next generation sequencing (Roche/454), a microarray hybridisation
station (Array Booster, Advalytix) with four independent chambers,
an hybridisation oven (DNA Microarray Oven, Agilent), a microarray
scanner (Microarray scanner, Agilent), a fluorescent microscope (ZEISS
Axioskop 40FL). Moreover, there are two cell culture rooms equipped
with a laminar hood, a CO2 incubator and a liquid
nitrogen apparatus and a −135°C freezer for cell lines storage.
Laboratory of
Biomedical Technologies and Experimental Oncology,
Department of Human Pathology and Oncology.
The laboratory,
located at ground floor
educational block of Policlinico S. Maria alle Scotte, viale Bracci
2 Siena, has approximately 247 sq. m. available, including laboratory spaces
(approximately 187 sq. m.) and three offices (approximately 28 sq.
m.). The laboratory spaces consist of a total of 18 workstations, a
cell-culture room and a bacterial-culture room.
The offices
include a total of 6 computer stations.
The laboratory is equipped with 3 sterile hoods (BIOAIR
INSTRUMENTS); 1 bacterial hood (HERON); 2 chemical hoods (HERON); 3
cell incubators (RS Biotech); 6 centrifuges (Sigma); 2 microscopes (ZEISS);
3 thermostatic baths (Fisher Brand); 3 PCR machines (MJ Research); 1
UV/Visible spectrophotometer (SHIMADZU); 1 Chemi-Doc molecular
imaging system (BIORAD); 1 shaker incubator (Thermo ELECTRON
CORPORATION); 1 fluorescence microscope (ZEISS); equipment for
nucleic acid and protein electrophoresis (BIORAD); 1 liquid nitrogen
apparatus for cell lines storage and a −80°C freezer.
ITT Core Research
Laboratory, Signal Transduction Unit
The laboratory is
located inside Toscana Life Sciences (TLS) Foundation, in a Science
Park positioned in Siena in the historical area of "Torre Fiorentina",
where Novartis Vaccines and Diagnostics Srl has its Research &
Development activities. The area also hosts the Molecular Biology
Department of Siena University. All together, these structures in
the campus offer state-of-the art technological platforms,
scientific equipment and support services. Inside the TLS Foundation
building the Unit has full access to: Equipped laboratory and office
space (80 mq) 6 workstations and reserved cell culture room. Reserved Rotor-Gene
6000 real-time rotary analyzer with high Resolution Melting
capability. Core Facility Instruments: Leica confocal microscope TCS
SP5, equipped with laser Ar 100 mW (458, 476, 488, 496, 514 nm),
HeNe 1 mW (543 nm), HeNe 2 mW (594 nm), HeNe 10 mW (633 nm) 50 mW
led (405 nm); NMR Varian 500 MHz; Spectrometer TSQ Quantum Access;
FACSCanto II BD; UV/VIS Spectrophotometer; Spectrometer 100
FT-INFRARED; Spectrofluorimeter LS55; MALDI TOF Ultraflex III TOF/TOF
200 system; P3 cell culture room.
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