Curriculum vitae of Prof. Gianni Pozzi

GIANNI POZZI, MD

Born in Siena, 1957. Italian citizen. Married since 1990.Two daughters, born in 1991 and in 1996.

CURRENT POSITION.

Full Professor of Microbiology, Univ. of Siena Medical School, from 1997.

EDUCATION.

“Maturità Classica”, a degree in liberal arts from Scuole Pie Fiorentine, Florence, 1974.

“Laurea in Medicina”,  Medical Degree from the Univ. of Siena Medical School, 1980.

“Specializzazione in Microbiologia”, Postgraduate Specialization School in Microbiology, University of Genoa, 1985.         

PAST POSITIONS.

· Research Associate, Duke Univ., North Carolina, 1982-85.

· Non-tenure Professor of Microbiology, Univ. of Siena, 1983-1986.

· Visiting Professor, The Rockefeller University, New York, 1987.

· Assistant Professor, Univ. of Verona, Univ. of Siena, and Univ. of Padua, 1987-1994.

· Full Professor of Microbiology, Univ. of Cagliari Medical School, 1994-1997.

 

PUBBLICAZIONI DEGLI ULTIMI SEI ANNI

Publications of the last six years

1.      *Oggioni M. R., Manganelli R., Contorni M., Tommasino M., Pozzi G.: Immunization of mice by oral colonization with live recombinant commensal streptococci. Vaccine, 13:775-779, 1995.

2.      Medaglini D., Pozzi G., King T.P., Fischetti V.A.: Mucosal and systemic immune responses to a recombinant protein expressed on the surface of the oral commensal bacterium Streptococcus gordonii after oral colonization. Proc. Natl. Acad. Sci. USA, 92:6868-6872, 1995.

3.      *Manganelli R., Romano L., Ricci S., Zazzi M., Pozzi G.: Dosage of Tn916 Circular intermediates in Enterococcus faecalis. Plasmid, 34:48-57, 1995.

4.      Oggioni M. R., Fattorini L., Li B., De Milito A., Zazzi M., Pozzi G., Orefici G., Valensin P.: Identification of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulare by selective nested polymerase chain reaction. Molecular and Cellular Probes, 9:321-326, 1995.

5.      Molina A. M., Palù G., Pozzi G., Valensin P.E.: Lo Streptococcus gordonii come vettore di vaccini ricombinanti. L’Igiene Moderna, 104:1-11, 1995.

6.      *Provvedi R., Manganelli R., Pozzi G.: Characterization of conjugative transposon Tn5251 of Streptococcus pneumoniae. FEMS Microbiol. Lett., 135:231-236, 1996.

7.      *Oggioni M. R., Pozzi G.: A host-vector system for heterologous gene expression in Streptococcus gordonii. Gene, 169:85-90, 1996.

8.      Oggioni M. R., Dowson C. G., Maynard-Smith J., Pozzi G.: The tetracycline resistance gene tet(M) exhibits mosaic structure. Plasmid, 35:156-163, 1996.

9.      Manganelli R., Ricci S., Pozzi G.: Conjugative transposon Tn916: evidence for excision with formation of 3’-protruding termini: J. Bacteriol, 178:5813-5816, 1996.

10.  Fischetti, V.A., Medaglini D., Pozzi G.: Gram-positive bacteria for mucosal vaccine delivery. Current Opinion in Biotechnology. 7: 659-666, 1996.

11.  *Pozzi G., Masala L., Iannelli F., Manganelli R., Håvarstein L. S., Piccoli L., Simon D., Morrison D.A.: Competence for genetic transformation in encapsulayted strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone. J. Bacteriol., 178:6087-6090, 1996.

12.  *Wells J.M., Pozzi G.: An overview of Gram-positive bacteria as Vaccine vehicles for mucosal immunization. In: G. Pozzi and J.M. Wells, eds. Gram-positive bacteria as vaccine vehicles for mucosal immunization. Biotechnology Intelligence Units, Springer-Verlag Berlin and Landes Bioscience Georgetown,  p.1-8, 1997.

13.  *Pozzi G., Oggioni M.R., Medaglini D.: Recombinant Streptococcus gordonii as a live vehicle for vaccine antigens. In: G. Pozzi and J.M. Wells, eds. Gram-positive bacteria as vaccine vehicles for mucosal immunization. Biotechnology Intelligence Units, Springer-Verlag Berlin and Landes Bioscience Georgetown,  p.35-60, 1997.

14.  *Rush C.M., Mercenier A., Pozzi G.: Expression of vaccine antigens in Lactobacillus. In: G. Pozzi and J.M. Wells, eds. Gram-positive bacteria as vaccine vehicles for mucosal immunization. Biotechnology Intelligence Units, Springer-Verlag Berlin and Landes Bioscience Georgetown, p.107-144, 1997.

15.  *Medaglini D., Rush C.M., Sestini P., Pozzi G.: Commensal bacteria as vehicles for mucosal vaccines against sexually transmitted diseases: vaginal colonization with recombinant streptococci induces local and systemic antibodies in mice. Vaccine, 15 :1157-1163, 1997.

16.  *Medaglini D., Ricci S., Maggi T., Rush C.M., Manganelli R., Oggioni M.R., Pozzi G.: Recombinant Gram-positive bacteria as vehicles of vaccine antigens. Biotechnology Annual Review, 3:297-312, 1997.

17.  Manganelli R., Ricci S., Pozzi G.: The joint of Tn916 circular intermediates is a homoduplex in Enterococcus faecalis. Plasmid, 38 :71-78, 1997.

18.  *Di Fabio S., Medaglini D., Rush C. M., Corrias F., Panzini G. L., Pace M., Verani P., Pozzi G., Titti F.: Vaginal immunization of cynomolgus monkeys (Macaca fascicularis) with Streptococcus gordonii expressing HIV-1 and HPV-16 antigens. Vaccine, 16 :485-492, 1998.

19.  Oggioni M. R., Gallieni P., Bigazzi C., Pozzi G., Valensin P.E. : Recurrent septicaemia in an immunocompromised patient due to probiotic strains of Bacillus subtilis. J. Clin. Microbiol., 36:325-326, 1998.

20.  Medaglini D., Oggioni M. R., Pozzi G. : Vaginal immunization with recombinant Gram-positive bacteria. Am. J. Reprod. Immunol., 39 :199-208, 1998.

21.  Rescigno M., Citterio S., Thèry C., Rittig M., Medaglini D., Pozzi G., Amigorena S., Ricciardi-Castagnoli P.: Bacterial-induced neo-biosynthesis, stabilization and surface expression of functional class I molecules in mouse dendritic cells. Proc. Natl. Acad Sci. USA, 95:5229-5234, 1998.

22.  *Iannelli F., Giunti L., Pozzi G.: Direct Sequencing of long polymerase chain reaction fragments. Molec. Biotech., 10, 183-5. 1998.

23.  Medaglini D., Maggi T., Ricci S., Oggioni M. R., Pozzi G. : Recombinant Streptococcus gordonii as a mucosal vaccine vector. Period. Biol., 100: 455-458, 1998.

24.  Medaglini D., Maggi T., Sestini P., Di Fabio S., Pace M., Titti F., Oggioni M. R., Pozzi G. : Mucosal vaccine delivery in the female genital tract by recombinant Gram-positive bacteria. Period. Biol., 100: 435-440, 1998.

25.  Manganelli R., Provvedi R., Berneri C., Oggioni M. R., Pozzi G. : Insertion vectors for construction of recombinant conjugative transposons in Bacillus subtilis and Enterococcus faecalis. FEMS Microbiol Lett. 1998; 168:259-68.

26.  Orrù G., Pozzi G.: Basi genetiche della resistenza alla rifampicina del Mycobacterium tuberculosis. Giornale Italiano di Chemioterapia, 45:73-76, 1998.

27.  Oggioni M. R., Iannelli F., Pozzi G.: Characterization of cryptic plasmids pDP1 and pSMB1 of Streptococcus pneumoniae. Plasmid. 1999; 41:70-2.

28.  Pozzi G., Meloni M., Iona E., Orrù G., Thoresen O. F., Ricci M. L., Oggioni M. R., Fattorini L., Orefici G. : rpoB Mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy. J Clin Microbiol 1999; 37:1197-9.

29.  Iannelli F., Pearce B. J., Pozzi G.: The type 2 capsule locus of Streptococcus pneumoniae. J. Bacteriol., J Bacteriol. 1999;181:2652-2654.

30.  Oggioni M. R., Medaglini D., Romano L., Peruzzi F., Maggi T., Lozzi L. , Bracci L., Zazzi M., Manca F., Valensin P. E., Pozzi G.: Antigenicity and immunogenicity of the V3 domain of HIV-1 gp120 expressed in Streptococcus gordonii. AIDS Res Hum Retroviruses. 1999; 15:451-9.

31.  Oggioni M. R., Medaglini D., Maggi T., Pozzi G. : Engineering of the Gram-positive bacterial cell surface for the construction of bacterial vaccine vectors. Methods, 19, 163-173, 1999.

32.  Corinti S., Medaglini D., Cavani A., Rescigno M., Pozzi G., Ricciardi-Castagnoli P., Girolomoni G.:  Human Dendritic cells very efficiently present a heterologous antigen expressed on the surface of recombinant Gram-positive bacteria to CD4+ T lymphocytes. J. Immunol., 163, 3029-3036, 1999.

33.  Fattorini L., Iona E., Ricci M. L., Thoesen O. F., Orrù G., Oggioni M. R., Tortoli E., Piersimoni C., Chiaradonna P., Tronci M., Pozzi G., Orefici G.: Activity of 17 antimicrobial agents against drug-resistant strains of Mycobacterium tuberculosis. Microbial Drug Resistance, in press, 1999.

34.  Ricci S., Medaglini D., Rush C. M., Marcello A., Peppoloni S., Manganelli R., Palù G., Pozzi G.: Immunogenicity of the B monomer of Escherichia coli heat-labile toxin expressed on the surface of Streptococcus gordonii. Infect. Immun., 68: 760-776, 2000.

35.  Corinti S, Medaglini D, Prezzi C, Cavani A, Pozzi G, Girolomoni G: Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii. Infect Immun., 68: 1879-1883, 2000.

36.  Maria R. Spinosa, Tiziana Braccini, Ezio Ricca, Maurilio De Felice, Lorenzo Morelli, Gianni Pozzi and Marco R. Oggioni (2000) On the fate of ingested Bacillus spores. Research in Microbiology.

37.  Santagati M., Iannelli F., Oggioni M.R., Stefani S., Pozzi G.: 2000.Characterization of a genetic element carrying the macrolide efflux gene mef(A) in Streptococcus pneumoniae. Antimicrob. Agents Chemoter., 44(9):2585-2587

38.  Maggi T., Oggioni M.R., Medaglini D., Biancgi Bandinelli M.L., Soldateschi D., Wiesmuller K-H., Muller C.P., Valensin P.E., Pozzi G.: Expression of measles virus antigens in Streptococcus gordonii.New Microbiologica. 23:119-128.2000

39.  Kelley C., Medaglini D., Younson S., Pozzi G.: Biotechnological approaches to fight pathogens at mucosal sites. Biotechnology & Genetic Engineering Reviews. 18: xx. in press

40.  Oggioni M.R., Beninati C., Boccanera M., Spinosa M.R., Maggi T., Conti S., Magliani W., De Bernardis F., Teti G., Cassone A., Pozzi G., and Polonelli L.: Recombinant Streptococcus gordonii for mucosal delivery of a scFv microbicidal antibody. International Reviews in Immunology. Manoscritto accettato per la pubblicazione.

41.  Beninati C, M.R.Oggioni , M.Boccanera , M.R.Spinosa , T.Maggi , S. Conti, W. Magliani, F. De Bernardis, G. Teti, A. Cassone, G. Pozzi, and L. Polonelli. 2000. Therapy of mucosal candidiasis by expression of an anti-idiotype in human commensal bacteria. Nature Biotechnology, 18(10):1060-1064.

42.  Talay S.R., A. Zock, M. Rohde, G. Molinari, M.R. Oggioni, G. Pozzi, C.A. Guzman, and G.S. Chhatwal. 2000. Cooperative binding of human fibronectin to SfbI protein triggers streptococcal invasion into respiratory epithelial cells. Cellular Microbiology, 2(4): in press

43.  Medaglini D., Ciabattini A., Spinosa M., Marcotte H., Oggioni M.R., Pozzi G.: Immunization with recombinant Streptococcus gordonii expressing tetanus toxin fragment C confers protection from lethal challenge in mice. Vaccine. Manoscritto accettato per la pubblicazione

44.  Orrù G., Iona E., Thoresen O. F., Oggioni M. R., Fattorini L., Orefici G., Pozzi G. : Intermediate resistance to rifampin in clinical strains of Mycobacterium tuberculosis. Microbial Drug Resistance.

45.  Janulczyk R., Iannelli F., Sjöholm A., Pozzi G. and L. Björck. 2000. - Hic, a novel surface protein of Streptococcus pneumoniae that interferes with complement function. The Journal of Biological Chemistry, Aug 30 [epub ahead of print]

46.  Pearce B., Iannelli F., and G. Pozzi. - New unencapsulated recipients for transformation of Streptococcus pneumoniae. Manuscript in preparation

47.  Iannelli F., Scarselli M., Bernini A., Oggioni M.R., Morrison D.A., Havarstein L.S., Niccolai N., and G. Pozzi. - On the specificity of pneumococcal competence-pheromone histidine kinase receptor. Manuscript in preparation

48.  Iannelli F., Oggioni M.R., Spinosa M.R., and G. Pozzi. - Allelic Variation in the highly polymorphic pspC locus of Streptococcus pneumoniae. Manuscript in preparation

49.  Iannelli F., and G. Pozzi. - A method for site-directed in frame deletion mutagenesis in encapsulated strains of Streptococcus pneumoniae. Manuscript in preparation

 

(*) Lavori dei quali GP è il “corresponding author”.