AP-1 Network Project

Curriculum Vitae

Name Michiel van der Sanden
Postal adres Dr. Struyckenstraat 21-B
Postcode & City 4812 BA, Breda
Telephone work 0049-6221424573
Telephone mobile 0031-627300954
Day of birth 8 January 1975
Gender Male
Nationality Dutch

Period 04-2004 till present
Employer Deutsches Krebsforschungszentrum Heidelberg (DKFZ),
Department of Signal-transduction & Growth control
Function Post-doc researcher
· Perform scientific research and writing articles
· Supervision college students and PhD students

Period 02-2000 till 04-2004
Employer Faculty of Veterinary Medicine, University of Utrecht
Department of Biochemistry and Cell biology
Function PhD student, obtained PhD in September 2004
· Perform scientific research and writing articles
· Supervision university college students
· Design lectures and teaching them to students Biomedical Sciences and Veterinary Medicine
· Present presentations on congresses

Period 02-1999 till 11-1999
Employer Hubrecht laboratory, Dutch Institute for Developmental Biology
Department of Molecular Cell biology
Function Specialisation phase of Master’s study Biology
· Perform scientific research

Period 03-1998 till 12-1998
Employer Faculty Biology, University of Utrecht
Department of Comparative Endocrinology
Function Specialisation phase of Master’s study Biology
· Perform scientific research

Period 03-2005
Education Introduction into Project-management, within the DKFZ

Period 06-2003
Education Course Animal Handling and Well-fare (Ex art. 9 Wet op de proefdieren), by the department of Animal Well-fare, Utrecht

Period 11-2002 till 12-2002
Education Course Radiation Safety, level 4B, by IRI, Delft

Period 03-2002 till 05-2002
Education Course writing and editing for the scientific author, by the Graduate School for Animal Health (GSAH), Utrecht

Period 09-1994 till 01-2000
Education University of Utrecht, Master’s degree Biology
Specialisation research phase: Cell biology, Endocrinology
Differentiation courses: Molecular Cell biology, Biological Toxicology, Hormonal Regulation, Animal Physiology and Comparative Physiology

Period 08-1987 till 06-1994
Education St. Oelbert Gymnasium, Oosterhout
Exam courses: Dutch, English, Latin, Biology, Mathematics A, Mathematics B, Chemistry and History

01-2005 Lars Hummerich, Regina Mueller, Michiel van der Sanden, Felix Kokocinski, Meinhard Hahn, Gerhard Fuerstenberger, Cornelia Mauch, Peter Lichter, Jochen Hess and Peter Angel. (2005) Differential expression of S100 family members in squamous skin carcinogenesis correlates with altered AP-1 and NFkB activation. Cancer Res. submitted

09-2004 Van der Sanden, M.H.M., Meems, H., Houweling, M., Helms J.B. and Vaandrager, A.B. (2004). Induction of CHOP/GADD153 expression during inhibition of phosphatidylcholine synthesis is mediated via activation of a C/EBP-ATF responsive element. J. Biol. Chem. [Epub ahead of print]

03-2004 Van der Sanden, M.H.M., Houweling, M., Duijsings, D., Vaandrager, A.B. and Van Golde, L.M.G. (2004). Inhibition of phosphatidylcholine synthesis is not the primary pathway in hexadecylphosphocholine-induced apoptosis. Biochim. Biophys. Acta. 1636, 99-107

04-2003 Van der Sanden, M.H.M., Houweling, M., Van Golde, L.M.G.and Vaandrager, A.B. (2003). Inhibition of phosphatidylcholine synthesis induces expression of the endoplasmic reticulum stress and apoptosis related protein C/EBP-Homologous Protein (CHOP/GADD153). Biochem. J., 369, 643-50.

03/2005 Posterpresentation on AEK/AIO Cancer Conference in Würzburg
Michiel van der Sanden, Lars Hummerich, Regina Mueller, Peter Lichter, Jochen Hess and Peter Angel (2005) The function of S100 proteins in the process of squamous skin carcinogenesis.

11/2003 Oral presentation on the IB AIO-day, Driebergen
Michiel van der Sanden (2003) Phospholipids and apoptosis.

03/2003 Oral presentation on the IB congress, Lunteren
Michiel van der Sanden (2003) Inhibition of PC synthesis induces expression of apoptotic CHOP/GADD153 through a specific ER-independent stress response.

01/2003 Posterpresentation on Apoptosis 2003, congress in Luxemburg
Michiel H.M. van der Sanden, Martin Houweling, Lambert M.G. van Golde and Arie B. Vaandrager (2003). Inhibition of phosphatidylcholine synthesis leads to a decrease in the diacylglycerol/ceramide ratio preceding apoptosis.

09/2002 Posterpresentation on 43rd International Conference on the Bioscience of Lipids (ICBL), congress in Graz
Michiel H.M. van der Sanden, Martin Houweling, Lambert M.G. van Golde and Arie B. Vaandrager (2002). PC depletion induces the expression of pro-apoptotic, ER stress related CHOP/GADD 153

07/2001 Posterpresentation on Gordon Research conference, congress in Boston Michiel H.M. van der Sanden, Martin Houweling, Lambert M.G. van Golde and Arie B. Vaandrager (2001). Phosphatidylcholine depletion induces apoptosis; possible involvement of the ER stress response

03/2001 Posterpresentation on IB congress in Lunteren. Winner IB posterprijs Michiel H.M. van der Sanden, Martin Houweling, Lambert M.G. van Golde and Arie B. Vaandrager (2001). Phosphatidylcholine depletion induces apoptosis; possible involvement of the ER stress response

organ isolation of laboratory animals, RNA isolation, RNA nothern blot hybridisation, Reverse Transcriptase Poly Chain Reaction (RT-PCR), Real time light cycler PCR (RQ-PCR), RNAi, In situ site-directed mutagenesis, DNA cloning, DNA fingerprinting, gel-electroforese, DNA sequencing, bacterial culture, cell culture, luciferase reporter assays, Elisa, protein co-immunoprecipitation, protein SDS-PAGE electrophoresis, 2D-Gel electrophoresis, western blot, ChIP analyses, EMSA, FACS, fluorescense microscopy, confocal-microscopy, electron-microscopy, lipid analysis by HPLC and mass-spectrometry, Two-Hybrid screening.

AP-1 Network Project

The function of S100 proteins in the process of squamous skin carcinogenesis
Michiel van der Sanden, Lars Hummerich, Regina Mueller, Ingeborg Vogt, Meinhard Hahn, Gerhard Fürstenberger, Cornelia Mauch, Peter Lichter, Jochen Hess and Peter Angel
The chemically induced protocol of skin carcinogenesis represents an extensively utilized animal model system to unravel the multistage nature of tumor development and to design novel therapeutic concepts. In an effort to define genetic programs characteristic for different stages of epithelial carcinogenesis, we combined this tumor model with global gene expression profiling on two independent cDNA microarrays. Application of bioinformatics on our data highlighted a significant correlation between specific members of the S100 gene family, located on human chromosome 1q21, and squamous skin carcinogenesis. The family members S100A6, S100A8 and S100A9 were highly increased at the different stages of mouse skin tumourigenesis. In contrast, another member of this family, S100A3 is strongly down-regulated in all stages of skin carcinogenesis. The purpose of this study is to identify the molecular processes by which these S100 proteins display their activity and thereby contribute to the process of squamous skin carcinogenesis. Functional analysis in epithelial cell lines revealed that the heterodimer of S100A8 and S100A9 modulate gene transcription via NFkB and AP-1, two essential transcription factors in neoplastic transformation of keratinocytes. To further characterize the function of S100 proteins in epithelial malignancy, we initiated the generation and characterization of genetically modified cell lines. The identification of S100 proteins as novel tumor-associated genes and their functional characterization might represent a highly promising tool to develop improved prognostic markers and strategies for chemoprevention and therapy in epithelial malignancy.

PROJECT

The function of S100 proteins in the process of squamous skin carcinogenesis
Michiel van der Sanden, Lars Hummerich, Regina Mueller, Ingeborg Vogt, Meinhard Hahn, Gerhard Fürstenberger, Cornelia Mauch, Peter Lichter, Jochen Hess and Peter Angel
The chemically induced protocol of skin carcinogenesis represents an extensively utilized animal model system to unravel the multistage nature of tumor development and to design novel therapeutic concepts. In an effort to define genetic programs characteristic for different stages of epithelial carcinogenesis, we combined this tumor model with global gene expression profiling on two independent cDNA microarrays. Application of bioinformatics on our data highlighted a significant correlation between specific members of the S100 gene family, located on human chromosome 1q21, and squamous skin carcinogenesis. The family members S100A6, S100A8 and S100A9 were highly increased at the different stages of mouse skin tumourigenesis. In contrast, another member of this family, S100A3 is strongly down-regulated in all stages of skin carcinogenesis. The purpose of this study is to identify the molecular processes by which these S100 proteins display their activity and thereby contribute to the process of squamous skin carcinogenesis. Functional analysis in epithelial cell lines revealed that the heterodimer of S100A8 and S100A9 modulate gene transcription via NFkB and AP-1, two essential transcription factors in neoplastic transformation of keratinocytes. To further characterize the function of S100 proteins in epithelial malignancy, we initiated the generation and characterization of genetically modified cell lines. The identification of S100 proteins as novel tumor-associated genes and their functional characterization might represent a highly promising tool to develop improved prognostic markers and strategies for chemoprevention and therapy in epithelial malignancy.